anti human cd107a antibody  (fluidigm)

Journal: JCI Insight

Article Title: Pregnancy and lactation induce distinct immune responses to COVID-19 booster vaccination and SARS-CoV-2 breakthrough infection

doi: 10.1172/jci.insight.191930

Figure Lengend Snippet: ( A ) Percentages of CD107a + Perforin + cells, CD107a + GzmB + cells, and GzmB + Perforin + cells among SARS-CoV-2–specific CD8 + T cells in pregnant (left) and lactating (right) participants of Study C. P values were calculated by Mann-Whitney U test or Welch’s t test, depending on normality and equality of variance testing. ( B ) The frequencies of cytolytic (CD107a + GzmB + ) SARS-CoV-2–specific CD8 + T cells positively associate with anti-RBD IgA titers following breakthrough infection in pregnant but not lactating individuals. ( C ) The frequencies of SARS-CoV-2–specific CD4 + Tem cells positively associate with anti-N IgG titers following breakthrough infection in lactating but not pregnant individuals. ( D ) The frequencies of SARS-CoV-2–specific CD4 + Treg cells negatively associate with anti-N IgG titers following breakthrough infection in lactating but not pregnant individuals. For all panels, individuals who were lactating for the duration of the study are indicated with open circles colored brown (vaccination group) and purple (breakthrough infection group). For all correlation plots, r indicates Pearson’s correlation coefficient, and P values were determined by Pearson’s correlation tests. Data from this figure correspond to that generated from n = 10 vaccine and n = 10 breakthrough participants in the pregnant group, and n = 18 vaccine and n = 7 breakthrough participants in the lactating group.

Article Snippet: 151 Eu-labeled anti–human CD107a antibody (Standard BioTools, 1 μL per 3 million cells) was added to all samples during the incubation to monitor degranulation.

Techniques: MANN-WHITNEY, Infection, Generated

Journal: ImmunoHorizons

Article Title: Characterization of immune phenotypes in peripheral blood of adult renal transplant recipients using mass cytometry (CyTOF)

doi: 10.1093/immhor/vlae013

Figure Lengend Snippet: CyTOF staining panel for phenotypic and functional analysis of immune cell subsets, with metal labels, antibody clone, and source.

Article Snippet: 151Eu , CD107a , H4A3 , Fluidigm.

Techniques: Staining, Functional Assay

Journal: Journal for Immunotherapy of Cancer

Article Title: Phase II basket trial of Dual Anti-CTLA-4 and anti-PD-1 blockade in Rare Tumors (DART) SWOG S1609: pancreatic neuroendocrine neoplasm (PNEN) cohort

doi: 10.1136/jitc-2025-011760

Figure Lengend Snippet: Increased cytotoxicity profiles in circulation associate with response. Cytometry by time-of-flight profiling of PBMCs after activation with PMA/ionomycin at baseline (base), cycle 2 week 9 (C2W9) and progression (prog) time points. Cell types are graphed as a proportion of total immune cells. Patients with response are shown in red (PR, solid red; SD>6 months, hashed red; n=1 each). Blue dots represent patients with PD or SD<6 months with a hashed line connecting paired longitudinal time points. ( A ) Changes in frequencies of major T-cell lineages (total T cells, CD4+T cells, CD8+T cells and Tregs) over time are shown with decreases in Tregs observed in PR patient. ( B ) Memory states in CD4+T cells (top row) and CD8+T cells (bottom row) show expansion of effector memory and TEMRA subsets in PR patient. ( C ) Cytotoxicity profile as determined by granzyme B (GZMB), perforin and interferon-γ production post stimulation within the CD4+effector memory T cells (EM; top row) and CD8+EM T cells (bottom row). Increased expression of granzyme B (GZM_B) and perforin is observed in PR patient. C2W9, course 2 week 9; EM, effector memory; PBMC, peripheral blood mononuclear cells; PMA, phorbol myristic acetate; PR, partial response; Prog, progression; SD, stable disease; TEMRA, CD45RA+T cells; Treg, regulatory T cell.

Article Snippet: After resting, secretion inhibitors brefeldin A (5 μg/mL) and monensin (5 μg/mL) (Millipore-Sigma, St. Louis, Missouri, USA) were added along with 10 ng/mL phorbol myristic acetate and 1 μg/mL ionomycin (Millipore-Sigma) and anti-CD107a conjugated with 151Eu.

Techniques: Cytometry, Activation Assay, Expressing

Journal: Cell Reports Medicine

Article Title: High-dimensional profiling of pediatric immune responses to solid organ transplantation

doi: 10.1016/j.xcrm.2023.101147

Figure Lengend Snippet: Antibodies used for CyTOF staining

Article Snippet: CD107a , 151-Eu , H4A3 , Standard Biotools , 3151002B.

Techniques:

Journal: Cell Reports Medicine

Article Title: High-dimensional profiling of pediatric immune responses to solid organ transplantation

doi: 10.1016/j.xcrm.2023.101147

Figure Lengend Snippet:

Article Snippet: CD107a , 151-Eu , H4A3 , Standard Biotools , 3151002B.

Techniques: Recombinant, Antibody Labeling, Staining, Blocking Assay, Software

Journal: Cell Reports Medicine

Article Title: High-dimensional profiling of pediatric immune responses to solid organ transplantation

doi: 10.1016/j.xcrm.2023.101147

Figure Lengend Snippet: Antibodies used for CyTOF staining

Article Snippet: CD107a , 151-Eu , H4A3 , Standard Biotools , 3151002B.

Techniques:

Journal: Cell Reports Medicine

Article Title: High-dimensional profiling of pediatric immune responses to solid organ transplantation

doi: 10.1016/j.xcrm.2023.101147

Figure Lengend Snippet:

Article Snippet: CD107a , 151-Eu , H4A3 , Standard Biotools , 3151002B.

Techniques: Recombinant, Antibody Labeling, Staining, Blocking Assay, Software